The prolyl isomerase FKBP11 is a secretory translocon accessory factor
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Structural analysis of the dynamic ribosome-translocon complex
Lewis, A.J.O., Zhong, F., Keenan, R.J. and Hegde, R.S. (2024) eLife, Jun 18;13. (doi: 10.7554/eLife.95814)
A unifying model for membrane protein biogenesis
Hegde, R.S. and Keenan, R.J. (2024) Nat Struct Mol Biol, 31:1009-1017. (doi: 10.1038/s41594-024-01296-5)
Substrate-driven assembly of a translocon for multipass membrane proteins
Sundaram, A.#, Yamsek, M.#, Zhong, F.#, Hooda, Y., Hegde, R.S. and Keenan, R.J. (2022)
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Mechanism of an intramembrane chaperone for multipass membrane proteins
Smalinskaitė, L.#, Kim, M.K.#, Lewis, A.J.O., Keenan, R.J., and Hegde, R.S. (2022)
Nature, 611:161-166. (#=equal contribution) (doi: 10.1038/s41586-022-05336-2)
The mechanisms of integral membrane protein biogenesis
Hegde, R.S. and Keenan, R.J. (2021) Nature Reviews Molecular Cell Biology. (doi: 10.1038/ s41580-021-00413-2)
An ER translocon for multi-pass membrane protein biogenesis
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ. (2020)
eLife, Aug 21;9:e56889. (doi: 10.7554/eLife.56889)
The architecture of EMC reveals a path for membrane protein insertion
O'Donnell JP, Phillips BP, Yagita Y, Juszkiewicz S, Wagner A, Malinverni D, Keenan RJ, Miller EA, Hegde RS. (2020)
eLife, May 27;9:e57887. (doi: 10.7554/eLife.57887)
A structural perspective on tail-anchored protein biogenesis by the GET pathway
Mateja, A and Keenan, RJ (2018) Current Opinion in Structural Biology, 51:195-202.
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Identification of Oxa1 homologs operating in the eukaryotic endoplasmic reticulum
Anghel, S.A., McGilvray, P.T., Hegde, R.S., Keenan, R.J. (2017) Cell Reports, 21:3708-3716.
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Tail-anchored protein insertion by a single Get1/2 heterodimer
Zalisko, B.E., Chan, C., Denic, V., Rock, R.S. and Keenan, R.J. (2017) Cell Reports, 20:2287-2293.
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Msp1 is a membrane protein dislocase for tail-anchored proteins
Wohlever ML, Mateja A, McGilvray PT, Day KJ and Keenan, RJ (2017) Molecular Cell, 67:194-202.
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The GET system inserts the tail-anchored SYP72 protein into endoplasmic reticulum membranes
Srivistava R, Zalisko BE, Keenan RJ, Howell SH. (2017) Plant Physiol., 173:1137-1145.
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Ubiquilins chaperone and triage mitochondrial membrane proteins for degradation
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Data publication with the structural biology data grid supports live analysis
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Conformational chaperones for structural studies of membrane proteins using antibody phage display
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A dual fluorescent reporter for the investigation of methionine mistranslation in live cells
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A YidC-like protein in the archaeal plasma membrane
Borowska, M.T., Dominik, P.K., Anghel, S.A., Kossiakoff A.A., Keenan R.J. (2015) Structure, 23:1715-1724.
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Structure of the Get3 targeting factor in complex with its membrane protein cargo
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Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12
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Tail-anchored membrane protein insertion into the endoplasmic reticulum
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The mechanism of membrane-associated steps in tail-anchored protein insertion
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A conserved archaeal pathway for tail-anchored membrane protein insertion
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Noncytotoxic DsRed derivatives for whole-cell labeling
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A ribosome-associating factor chaperones tail-anchored membrane proteins
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Chromophore formation in DsRed occurs by a branched pathway
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The structural basis of tail-anchored membrane protein recognition by Get3
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A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling
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Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling
Strack, R.L., Bhattacharyya, D., Glick, B.S., Keenan, R.J. (2009) BMC Biotech., 9:32.
A noncytotoxic DsRed variant for whole-cell labeling
Strack, R.L., Strongin, D.E., Bhattacharyya, D., Tao, W., Berman, A., Broxmeyer, H.E., Keenan, R.J., Glick, B.S. (2008)
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Spectral diversity of fluorescent proteins from the anthozoan Corynactis californica
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Structural rearrangements near the chromophore influence the maturation speed and brightness of
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The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase
Siehl, D.L., Castle, L.A., Gorton, R., Keenan, R.J. (2007) J. Biol. Chem., 282:11446-11455.
DNA Shuffling as a tool for protein crystallization
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Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy
Siehl, D.L., Castle, L.A., Gorton, R., Chen, Y.H., Bertain, S., Cho, H.J., Keenan, R.J., Liu, D., Lassner, M.W. (2005)
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Laboratory-Directed Protein Evolution
Yuan, L., Kurek, I., English, J., Keenan, R.J. (2005) Microbiol. Mol. Biol. Rev., 69:373-392.
Nontransgenic crops from transgenic plants
Keenan, R.J., Stemmer, W.P. (2002) Nature Biotech., 20:215-216.
The Signal Recognition Particle
Keenan, R.J., Freymann, D.M., Stroud, R.M., Walter, P. (2001) Annu. Rev. Biochem., 70:755-775.
Perspectives: structural biology. SRP – where the RNA and membrane worlds meet
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Structure of the phylogenetically most conserved domain of SRP RNA
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Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+-GDP
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Crystal Structure of the Signal Sequence Binding Subunit of the Signal Recognition Particle
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Structure of the conserved GTPase domain of the signal recognition particle
Freymann, D.M., Keenan, R.J., Stroud, R.M., Walter, P. (1997) Nature, 385:361-364.
NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP)
Schmitz, U., Freymann, D.M., James, T.L., Keenan, R.J., Vinayak, R., Walter, P. (1996) RNA, 2:1213-1227.
Rutenber, E., Fauman, E.B., Keenan, R.J., Fong, S., Furth, P.S., Ortiz de Montellano, P.R., Meng, E., Kuntz, I.D.,
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Keenan Lab UChicago